Composite

Part:BBa_I714051:Design

Designed by: Mingzhi Qu   Group: iGEM07_Peking   (2007-10-20)


[I714030][J23066][I714030]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 278
    Illegal NheI site found at 324
    Illegal NheI site found at 985
    Illegal NheI site found at 1031
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 484
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 361


Design Notes

Simply ligate the three parts sequentially to test the expression after tandem oriT is truncated.

Source

[I714030] + [J23066] + [I714030]

References